Phospho explorer antibody array

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WebMar 18, 2024 · The Phospho Explorer Antibody Array kit used in this study was obtained from Full Moon BioSystems (catalog PEX100; Sunnyvale, CA, USA) and covered 1318 well-characterized antibodies, the phosphorylated and non-phosphorylated state of these proteins will be detected at the same time (Pulito et al. 2016 ). WebAug 3, 2024 · A phospho-protein array analysis revealed that PF-3758309 down-regulates the NF-κB signaling pathway, which provides the most likely mechanism by which PF-3758309 inhibits latency reversal.

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WebAKT Phospho-Specific Array includes 137 highly specific and well-characterized phosphorylation antibodies in the AKT pathway. Product detail AKT/PKB Signaling Phospho-Specific Array grand marais mn things to do

Example Protocol for Protein Arrays - BioCat

WebNov 14, 2024 · The Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and biological processes. Web46 rows · The Proteome Profiler Human Phospho-Kinase Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Phosphorylation of captured proteins is detected with biotinylated phospho-specific detection antibodies … WebMoon BioSystems’ antibody arrays allow researchers to detect and analyze hundreds of proteins simultaneously on a single slide, saving precious resources and reducing the number of variables that affect experimental outcome. Our unique collection of antibody arrays includes phospho-specific arrays grand marais north shore health

Proteome Profiler Human Phospho-Kinase Array Kit ARY003B

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WebHuman Protein S-Nitrosylation Array G2 The RayBiotech G-Series Human Protein S-Nitrosylation Antibody Array 2 is a multiplex assay on glass slide for the relative detection of cystein s-nitrosylation of 493 different human proteins cell lysate, culture supernatant, serum, plasma and other biological samples. WebWe used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. We identified CDK5, Src, and P38MAPK as key downstream … grand marais mn web live cameraWebThe Human Phospho-Kinase Array (Catalog # ARY003B) is a rapid, sensitive, and economical tool to simultaneously detect the relative site-specific phosphorylation of 43 kinases and 2 related total proteins without performing numerous immunoprecipitations and Western blots. grand marais mn weather 10

"WebFeb 12, 2024 · Phospho-protein array Screening of 1318 phospho-proteins (Phospho Explorer Antibody Array #KAS02) was performed in hippocampal neurons under two experimental conditions: vehicle and 100 nM... " - Phospho explorer antibody array

Phospho explorer antibody array

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WebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). WebPhospho Explorer Antibody Array data of KGN cells treated with Hu‐17. (A) Heatmap of proteins differently phosphorylated proteins in KGN cells with Hu‐17 treatment. The data included in the heatmap is beyond cutoff line with the change fold is ≤77% or ≥130%. A is the control group, B is the Hu‐17 treated for 3 h group, C is the Hu ...

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WebApoptosis Phospho Antibody Array This array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study serine/threonine and tyrosine phosphorylation at specific sites. WebTo understand the role of phosphoproteomic signaling in carvedilol's anticancer activity, the mouse epidermal JB6 P+ cells treated with EGF, carvedilol, or their combination were analyzed using the Phospho Explorer Antibody Array containing 1318 site-specific and phospho-specific antibodies of over 30 signaling pathways.

WebMar 25, 2016 · Phospho-protein profiling by Phospho Explorer Antibody Array. Cell lysates obtained from PC-1 and PC-1.0 cells were applied to a Phospho Explorer Antibody Array (Full Moon Biosystems, Sunnyvale, CA, USA). The array contained 1318 antibodies. Each of the antibodies has two replicates that are printed on coated glass microscope slide, along … WebThe antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. Learn more about cytokine …

WebSep 1, 2024 · Total RNA was extracted by chloroform and isopropanol precipitation. cDNA was obtained using the SuperScript III First-Strand Synthesis System (Invitrogen, 18080-051). qPCR analyses were performed with primers listed below using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725270) in at least duplicate in three … Web2 Citations. Detects 55 phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types but intended for use with cell and tissue lysates. MANUAL / SDS. $901.00. Size. In stock. SKU. AAH-PPP-1-2.

WebMay 13, 2024 · Phosphodiesterase type 5 (PDE5) inhibitors are the most commonly used first-line treatment options for ED; however, these agents are ineffective in ~30% of patients and ultimately cannot rescue...

WebPhospho Explorer Array is a glass-based Array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples. It is a high-throughput ELISA based antibody microarray for qualitative/semi-quantitative protein … grand marais phone bookWebApr 29, 2024 · Phosphoprotein profiling by the Phospho Explorer Antibody Array PEX100, which was designed and manufactured by Full Moon Biosystems, Inc. (Sunnyvale, CA), contains 1318 antibodies. Each of the antibodies has two replicates that are printed on coated glass microscope slide, along with multiple positive and negative controls. grand marais pharmacyWebMay 6, 2014 · Several phosphokinase antibody arrays have been developed , including Phospho Explorer Array (Full Moon BioSystems), Signal Transduction AntibodyArray (Hypromatrix), Kinex Antibody Microarray (Kinexus Bioinformatics), Human Phospho-Kinase Antibody Array (R&D system), and Panorama Antibody Array XP725 (Sigma-Aldrich). grand marais national parkWebarray antibody pairs are optimised to provide the most accurate and reliable data. Human Cytokine Array Panel A (Cat# ARY005B) PBMCs untreated (top) or treated with ... p27/Kip1, PON2, Phospho-p53 (S15), Phospho-p53 (S46), Phospho-p53 (S392), Phospho-Rad17 (S635), SMAC/Diablo, Survivin, TNF RI/TNFRSF1A, TRAIL R1/DR4, TRAIL R2/DR5, XIAP ... grand marais mn to rochester mnWebSep 20, 2024 · The anti-ERK1/2 antibody was designed against the epitopes with the same phospho-sites as those detected by the phospho-array. All FFPE samples showed strong immunoreactivity for ERK1/2, with diffuse or regional positivity, and these results were in accordance with the very high relative phosphorylation of ERK2 as detected by the … chinese food nesconset nyWebMay 6, 2014 · Several phosphokinase antibody arrays have been developed , including Phospho Explorer Array (Full Moon BioSystems), Signal Transduction AntibodyArray (Hypromatrix), Kinex Antibody Microarray (Kinexus Bioinformatics), Human Phospho-Kinase Antibody Array (R&D system), and Panorama Antibody Array XP725 (Sigma-Aldrich). grand marais pharmacy windsorWebOne of the best selling antibody arrays with 269 key phospho-specific and site-specific antibodies from multiple cancer signaling pathways. Cytokine Profiling Array Featuring 310 cytokine related antibodies. Each antibody is printed with six replicates. Ideal for cytokine profiling and inflammation studies. Phospho Explorer Array chinese food neptune nj