Botrocetin
WebBotrocetin and vWF form a soluble complex. 4 publications Structure Features Showing features for beta strand, helix, turn. Expand table 3D structure databases AlphaFoldDB P22030 SMR P22030 ModBase Search… PDBe-KB Search… Miscellaneous EvolutionaryTrace P22030 Family & Domains Features Showing features for domain. … WebSnake venom from Bothrops jararaca mainly contains three types of toxins snake venom metalloproteinases (SVMP), snake venom serine proteases (SVSP), and C-type lectin …
Botrocetin
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WebNoun [ edit] botrocetin ( uncountable ) ( biochemistry) A coagglutinin, present in snake venom, that causes platelet aggregation This page was last edited on 28 July 2016, at 15:44. Text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. WebPubMed
WebThe venom botrocetin binds to the VWF A1 domain, potentiating its interaction with the GPIbα chain [ 11 ]. Upon vascular damage or atherosclerotic plaque rupture, collagen-bound VWF, in a shear-dependent way, initiates thrombus formation through a fast onset of GPIb-V-IX-binding and platelet adhesion [ 12, 13, 14 ]. WebFeb 1, 1993 · Two-chain botrocetin is a heterodimer composed of the alpha subunit (consisting of 133 amino acid residues) and the beta subunit (consisting of 125 amino …
WebNov 5, 2024 · Botrocetin has also been widely used experimentally for the development and characterization of potential antithrombotic drugs targeting the GPIb-VWF axis.
WebJan 1, 2001 · The monoclonal antibody 5D2 inhibits ristocetin- and botrocetin-dependent interactions and asialo-vWF dependent platelet aggregation, whereas CR1 inhibits only …
WebVWF TASP was found to inhibit specifically botrocetin‐induced platelet aggregation and to bind both botrocetin and GPIbα. However, in contrast to the native A1 domain, VWF TASP did not bind simultaneously to both ligands. Modeling studies revealed that the relative orientation of the α helices in VWF TASP led to a clash of bound botrocetin ... earned income credit 2022 max incomeTo show that botrocetin cannot form stable adhesions with platelets in the absence of the A1 domain, we evaluated platelet binding to surface-immobilized botrocetin under static conditions. After an incubation period of 10 min, few platelets remained attached upon the application of flow (Fig. 4a). By contrast, >50% … See more To shed light on the ability of botrocetin to 'activate' vWF such that it enhances its interaction with platelets, we first determined the crystal structure of the mouse A1–botrocetin … See more To determine the structure of the human ternary complex, we expressed the vWF-A1 binding domain of GPIbα using recombinant … See more The rigid-body movement of botrocetin in the ternary complex involves repacking of side chains at the A1-botrocetin interface (Fig. 3a,b). Two out of five salt bridges (using a distance cutoff of 3.5 Å) that occur within this … See more Comparison of the human ternary complex with the human A1–botrocetin binary complex reveals a marked rigid-body slide and rotation of … See more c svr crispr grna activityWebIt has been reported that botrocetin, a Bothrops venom factor, induces platelet aggregation dependent on von Willebrand factor (vWF), and that platelet aggregation induced by … earned income credit agi limit 2022WebJun 22, 2011 · Botrocetin engages vWF through two alpha helices (α5 and α6) on the exposed surface of the folded A1 domain [44]; in human and mouse models, site-directed mutagenesis studies have identified 12 sites distributed across these helices that are directly involved in botrocetin binding [47] ( Fig. 3a ). earned income credit amounts 2022WebNov 5, 2024 · Botrocetin inhibited platelet aggregation and thrombus formation in VWF-deficient mice. Conclusion: Integrin αIIbβ3 is the alternative receptor that mediates VWF-dependent, GPIb-independent... csv reader c++ githubWebOct 15, 2005 · Botrocetin/VWF-induced signaling through GPIb-IX-V produces TxA2 in an alphaIIbbeta3- and aggregation-independent manner. Binding of von Willebrand factor … earned income credit amount 2016WebMar 1, 1998 · Both mutations are phenotypically distinct from the previously reported variant type 2MMilwaukee-1 because of the presence of normal botrocetin-induced platelet binding, collagen binding, and AvW-3 binding, as well as the greater frequency and intensity of clinical bleeding. earned income credit 2023 for seniors